How does a chiral column work?

Chromatographic column chromatography is a variant of column chromatography in which the stationary phase contains a single enantiomer of a chiral compound rather than an achiral. … As with all chromatographic methods, the different stationary phases are particularly suitable for certain types of analyzers.

Chromatographic column chromatography is a variant of column chromatography in which the stationary phase contains a single enantiomer of a chiral compound rather than an achiral. The two enantiomers of the same analyte differ by affinity from the mono-enantiomeric stationary phase and then leave the column at different times.

The chiral stationary phase can be prepared by attaching a suitable chiral compound to the surface of an achiral support such as silica gel, which creates a chiral stationary phase (CSP). Many common chiral stationary phases are based on oligosaccharides such as cellulose or cyclodextrin (especially β-cyclodextrin, a seven-ring sugar molecule). As with all chromatographic methods, the different stationary phases are particularly suitable for certain types of analysis.

The chiral stationary phases are much more expensive than the comparable achiral stationary phases, such as C18.

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